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KMID : 1025520040460060917
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Journal of Animal Science and Technology
2004 Volume.46 No. 6 p.917 ~ p.924
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Genetic Variation and Population Specific Mitochondrial DNA Haplotype Found in the Jeju Native Pig Population
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Han Sang-Hyun
Cho In-Cheol
Lee Chong-Eon
Lee Sung-Soo
Kang S. R.
Choi Y. L.
Oh W. Y.
Seong Pil-Nam
Ko S. B.
Oh Moon-You
Ko Moon-Suck
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Abstract
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Using PCR-RFLP haplotyping for the mitochondrial DNA(mtDNA) fragment containing the NADH dehydrogenase 2 gene(ND2) and three tRNA genes(tRNA-Met, tRNA-Trp and tRNA-Ala), we characterized the genetic diversity of five pig breeds including Jeju native pigs. mtDNA polymorphisms showing distinct cleavage patterns were found in the pig breeds. Two digestion patterns were detected when HaeIII- and HinfI-RFLP, and four in the Tsp509I-RFLP analyses. Combining the three restriction enzyme digestion patterns found in five different pig breeds, four mtDNA haplotypes were observed and the haplotype frequencies were significantly different by the pig breeds. A monomorphic haplotype, mtWB, was observed in both Korean wild boars and Large White pigs. Both Duroc and Landrace pigs contained two haplotypes suggesting their multiple maternal lineages. Jeju native pig has two haplotypes(mtJN and mtJD). Of these, mtJN is identified as a Jeju native pig specific haplotype. This study suggested that more than two progenitor populations have been taken part in the domestication process of the Jeju native pig population, and/or probably subsequent crossing with other pig breeds from near east Asia. Unlike with our prediction, there was no direct evidence under molecular levels on the maternal introgression of Korean wild boar in the domestication of Jeju native pigs. In conclusion, specificity of mtDNA haplotypes related to pig breeds will be useful for identifying the maternal lineage as well as constructing the genealogical pedigree in pigs.
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KEYWORD
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Haplotype, Jeju native pig, Maternal origin, mtDNA, PCR-RFLP
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